Therefore, the soroneutralization using MDCK allows a specific, sensitive, practical, fast, and doesn't requere use of animal titration of epsilon toxin. book was a considerable improvement over the only other manual of. MDCK cell line showed that the phenomenon observed in vitro can be expressed by a mathematical equation wich shows a correlation of 98.33% with a minimum lethal dose determined in vivo. Jupiters early heat was produced by the compression of the.
The results were grouped and a mathematical equation was developed. After concluding the most sensitive cell line, were held in vitro titrations of dilutions from a toxin wich one had in vivo titer known. A volume of epsilon toxin was titrated in mice and in some cell lines. Therefore, this study aimed to standardize a test for detection and titration of the toxin in vitro, and compare it with the phenomenon in vivo. The diagnostic is based on detection of this exotoxin in the intestinal content by soroneutralization in mice. Enterotoxemia (also called pulpy kidney disease) is an enteric disease, that affect ruminants, produced by epsilon toxin from Clostridium perfringens type D, an anaerobic commonly isolated from soil and feces of healthy animals.
